Using Continuous Immersion System Liquid Media for In Vitro Microtuber Potato Production in Bioreactor

Purpose of this study was to determine effects of in vitro continuous immersion culture system, solid media, different doses of Thidiazuron (TDZ) (0.5, 1.0, 1.5 and 2.0 mg/l) and I Indole-3-butyric acid (IBA) (0.5, 1.0, 1.5 and 2.0 mg/l) on stem segments, microtuber number per plant. In vitro continuous immersion liquid culture media and solid media were used in potato (Solanum tuberosum L.) cv. Granola for stem segments and microtuber micropropagation. The stem segments were cultured on 1/2 Murashige and Skoog (MS) medium supplemented with 2 mg/l gibberellic acid, 10 mg/l paclobutrazol, 5.0 g/l activated charcoal, 100 g/l sucrose with or without 3.5 g/l phytagel; and TDZ and IBA in vitro. Liquid medium was distributed to the carrier in vitro continuous immersion with supporting net liquid culture system containing glass wool + filter paper layer as substrate. In vitro continuous immersion liquid culture media was more suitable and stable for organogenesis of potato microtubers than phytagel (3.5 g/l) solid media. After 30-day of incubation, there were 4.76 axillary stem segments formation having 7.21 mm diameter of microtubers weighing 155.74 mg fresh weight. Microtuber formation rate 84.66% and there were 2.06 microtubers per plant at 0.5 mg/l IBA in vitro continuous immersion liquid culture media treatment. 1.5 mg/l TDZ treatment had 7.56 axillary stem segments formation having 6.21 mm diameter of microtuber weighing 150.10 mg fresh weight. Microtuber formation rate was 92.55 % and there were 3.82 microtubers per plant. Formation and development of microtubers was lowest at 2.0 mg/l and highest at 1.5 mg/l TDZ concentration. At 1.5 ppm TDZ concentration, microtubers cropped from in vitro continuous immersion liquid culture system were bigger and heavier than phytagel solid media.